Figure 6
From: Distinct CD16a features on human NK cells observed by flow cytometry correlate with increased ADCC
IgG affinity profiling of primary human NK cells. (A) Flow cytometry plots of primary NK cell donors showing decreased 3G8 staining for kifunensine (20 µM) cells after incubation with 10% human serum, (B) a plot of the mean fluorescence intensities (MFIs) from (A) showing increased B73.1 staining and decreased 3G8 staining associated with high affinity IgG binding following kifunensine treatment, (C) representative flow cytometry plots of primary NK cell donors of healthy adults and children showing considerable heterogeneity, with high affinity phenotype (44%) observed in adult population and less percentage in children population (20%), (D) MFIs for the B73.1+ and 3G8+ population plotted against a heavy black y = x line with NK cells exhibiting greater antibody-binding affinity expected above this line.
