Figure 3

Anti-obesity effect of Zj7 on high fat diet induced obese (DIO) mice and db/db mice. Twelve-week-HFD-fed obese mice (A–G) were daily treated with vehicle or Zj7 (50 mg/kg) intraperitoneally (i.p.) for two weeks. (A) Body weight (g) (n = 10 for each group) and (B) Daily food intake of DIO mice for 12 days. (C) Average food consumption of DIO mice during 5–12 days of treatment. (D) Fat content normalized by lean mass (%) and (E) Lean mass (g) of DIO mice after two weeks of treatment (n = 10 for each group). (F) Serum leptin levels of vehicle (n = 10) or Zj7 (n = 8; 50 mg/kg) treated DIO mice after two weeks treatment. (G) Twelve-week-HFD-fed obese mice were fasted for sixteen hours then, treated with vehicle or Zj7 (50 mg/kg) i.p. and fed with food. Food intake after refed (g). (H–J) Six-week-old male db/db mice were treated with vehicle or Zj7 (50 mg/kg) for sixteen days (daily, i.p). (H) Body weight (g). (I) Daily food intake of db/db mice for 16 days. (J) Average food consumption of db/db mice during the treatment. (vehicle n = 9, Zj7 n = 10). Data are mean ± SEM. Statistical differences were determined by Student’s t-test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and ‘ns’ indicates not significant.