Figure 7

(A) Gene expression analysis for Bmm mRNA expression in body samples of Canton S10 (CS) or Dahomey (Dah) flies, 2 days (D2) or 7 days (D7) post eclosion in male and female flies. Gene expression was normalized using ribosomal genes as reference genes (RpL32/RpS13), βTub97EF/LaminCa or βTub60D/βTub97EF/LaminCa. Two-way ANOVA main effects as following: normalization; p < 0.0001; sex p < 0.0001 and their interaction; p = 0.003 with a Sidak’s multiple comparisons test comparing Bmm expression between males and females. ****p < 0.0001, *p < 0.05. (B) Gene expression analysis for dIlp6 mRNA expression in body samples of Canton S10 (CS) or Dahomey (Dah) flies, 2 days (D2) or 7 days (D7) post eclosion in male and female flies. Gene expression was normalized using ribosomal genes as reference genes (RpL32/RpS13), βTub97EF/LaminCa or βTub60D/βTub97EF/LaminCa. Two-way ANOVA main effects as following: normalization; p < 0.0001; sex p < 0.0001 and their interaction; p < 0.0001 with a Sidak’s multiple comparisons test comparing dIlp6 expression between males and females. ****p < 0.0001, *p < 0.05. (C) Gene expression analysis for fit mRNA expression in body samples of Canton S10 (CS) or Dahomey (Dah) flies, 2 days (D2) or 7 days (D7) post eclosion in male and female flies. Gene expression was normalized using ribosomal genes as reference genes (RpL32/RpS13), βTub97EF/LaminCa or βTub60D/βTub97EF/LaminCa. Two-way ANOVA main effects as following: normalization; p < 0.0001; sex p < 0.0001 and their interaction; p = 0.0008 with a Sidak’s multiple comparisons test comparing fit expression between males and females. ****p < 0.0001, ***p < 0.0005 *p < 0.05. (D) Gene expression analysis for Foxo mRNA expression in body samples of Canton S10 (CS) or Dahomey (Dah) flies, 2 days (D2) or 7 days (D7) post eclosion in male and female flies. Gene expression was normalized using ribosomal genes as reference genes (RpL32/RpS13), βTub97EF/LaminCa or βTub60D/βTub97EF/LaminCa. Two-way ANOVA main effects as following: normalization; ns, sex p < 0.0001 and their interaction; ns, with a Sidak’s multiple comparisons test comparing foxo expression between males and females.