Figure 10
From: OPN silencing reduces hypoxic pulmonary hypertension via PI3K-AKT-induced protective autophagy
Quantitative expression of autophagosomes and autophagy-related proteins in PASMCs. (A) Electron microscopic analysis (scale bar: 2 μm) of the number of autophagosomes (yellow uparrow) in the indicated groups. (B,C) Immunofluorescence (scale bar: 50 μm) showed the fluorescence expression intensity of LC3B and Beclin1 in PASMCs. (D–E) Quantification of LC3B and Beclin1 immunofluorescence. PASMCs in all groups were incubated in normoxic (5% CO2 and 20% O2) or hypoxic (5% CO2 and 1% O2) environments for 48 h. Results are representative of 3 independent experiments. Asterisks indicated statistically significant differences. ***p < 0.001.
