Figure 11
From: OPN silencing reduces hypoxic pulmonary hypertension via PI3K-AKT-induced protective autophagy
Under hypoxia, OPN and PI3K inhibition prevented PASMCs growth. (A) EdU staining of PASMCs showed alterations in cell proliferation (scale bar: 50 μm). (B) Flow cytometry analysis of the cell cycle. To calculate the percentage of cells in each phase, each set of cells was grown in the appropriate environment for 48 h before staining with PI. (C) Statistical chart showing the proportion of cells that were EdU-positive. (D) A plot of cell cycle dispersion distribution based on flow cytometry analysis. PASMCs in all groups were incubated in normoxic (5% CO2 and 20% O2) or hypoxic (5% CO2 and 1% O2) environments for 48 h. Results are representative of 3 independent experiments. Asterisks indicated statistically significant differences. ***p < 0.001.
