Figure 8
From: OPN silencing reduces hypoxic pulmonary hypertension via PI3K-AKT-induced protective autophagy
Inhibition of OPN in mouse lung tissue affected the expression of PI3K, LC3B, and Beclin1. (A) Relative OPN mRNA expression in lung tissues. (B) Relative PI3K mRNA expression in lung tissues. (C) Relative LC3B mRNA expression in lung tissues. (D) Relative Beclin1 mRNA expression in lung tissues. (E) OPN, PI3K, LC3B, and Beclin1 were analyzed by WB in the normoxia, normoxia + OPNfl/fl-TAGLN-Cre, hypoxia, and hypoxia + OPNfl/fl-TAGLN-Cre groups. (F–I) OPN, PI3K, LC3B, and Beclin1 proteins relative expression in the indicated groups. Results are representative of 3 independent experiments. Asterisks indicated statistically significant differences. **p < 0.01, ***p < 0.001.
