Figure 6

Data points represent the percentage Variable Allele Frequency (VAF) of the individual mitochondrial single nucleotide variants (mtSNVs). The error bars depict the mean allele frequency and 95% CI. (a) There was a significant difference in median heteroplasmy between individual nanobiopsy lysate samples (p = 0.004, Kruskal–Wallis test). (b) Although a difference in heteroplasmy between nanobiopsy samples was observed, this did not reach statistical significance (p = 0.054, Kruskal–Wallis test). (c) Biopsies were taken from three subcellular foci: Perinuclear (PN; n = 2, mean = 40.2, 95% CI = 27.6–52.9; mean median = 28.8), subsarcolemmal (SS; n = 3, mean = 40.6, 95% CI = 27.9–53.3; mean median = 7.5) and intermyofibrillar (IMF; n = 6, mean = 38.3, 95% CI = 29.1–47.5; mean median = 24.4). No significant difference in heteroplasmy between the different foci was observed (p = 0.555, Kruskal–Wallis test).