Table 1 Quad-immunofluorescence primary and secondary antibodies.
From: Nanobiopsy investigation of the subcellular mtDNA heteroplasmy in human tissues
Target | 1o antibody | Product code | Supplier | Dilution | 2o antibody | Product code | Supplier | Dilution |
|---|---|---|---|---|---|---|---|---|
Myonuclei | DAPI | D9542 | Sigma-Aldrich | 1:400 | – | – | – | – |
NDUFB8 | Mouse anti-NDUFB8 (IgG1) | ab110242 | Abcam | 1:100 | Biotin Anti-Mouse IgG1; | S32357 | Life technologies | 1:200 |
Strepavidin Alexa Fluor 647 | S21374 | Life technologies | 1:100 | |||||
MT-CO1 | Mouse anti-MT-CO1 (IgG2a) | ab14705 | Abcam | 1:100 | Alexa Fluor 433 Anti-Mouse IgG2a | S21131 | Life technologies | 1:200 |
VDAC1 | Mouse anti-VDAC1 (IgG2b) | ab14734 | Abcam | 1:100 | Alexa Fluor 546 Anti-Mouse IgG2b | A21143 | Life technologies | 1:200 |
