Table 3 Interobserver agreement between two pathologists in evaluating the TPS of IHC images, IF images with medium-level exposure time, and HDR images.

From: Optimizing immunofluorescence with high-dynamic-range imaging to enhance PD-L1 expression evaluation for 3D pathology assessment from NSCLC tumor tissue

 

IHC

IF-MED

HDR

All cases (n = 28)

26 (92.9%)

17 (60.7%)

21 (75%)

Weighted kappa

0.91

0.39

0.69

Interobserver reliability

Almost perfect

Fair

Substantial

  1. IHC immunohistochemistry, IF immunofluorescence, MED medium-level exposure time, HDR high dynamic range, TPS tumor proportion score.
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