Figure 2 | Scientific Reports

Figure 2

From: Biochemical indicators, cell apoptosis, and metabolomic analyses of the low-temperature stress response and cold tolerance mechanisms in Litopenaeus vannamei

Figure 2

TUNEL assay for hepatopancreatic cells apoptosis in cold tolerant (Lv-T) and common (Lv-C) shrimp under 28 °C and 10 °C. Under UV light, the DAPI-stained nuclei display blue, and the FITC-labeled positive apoptotic nuclei appear green. (A) DAPI-stained nuclei in Lv-C shrimp at 28 °C. (B) FITC-labeled nuclei in Lv-C shrimp at 28 °C. (C) Merge of DAPI-stained and FITC-labeled nuclei in Lv-C shrimp at 28 °C. (D) DAPI-stained nuclei in Lv-C shrimp at 10 °C. (E) FITC-labeled nuclei in Lv-C shrimp at 10 °C. (F) Merge of DAPI-stained and FITC-labeled nuclei in Lv-C shrimp at 10 °C. (G) DAPI-stained nuclei in Lv-T shrimp at 28 °C. (H) FITC-labeled nuclei in Lv-T shrimp at 28 °C. (I) Merge of DAPI-stained and FITC-labeled nuclei in Lv-T shrimp at 28 °C. (J) DAPI-stained nuclei in Lv-T shrimp at 10 °C. (K) FITC-labeled nuclei in Lv-T shrimp at 10 °C. (L) Merge of DAPI-stained and FITC-labeled nuclei in Lv-T shrimp at 10 °C.

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