Figure 4 | Scientific Reports

Figure 4

From: Icariin alleviates cisplatin-induced premature ovarian failure by inhibiting ferroptosis through activation of the Nrf2/ARE pathway

Figure 4

ICA reduces oxidative stress and ferroptosis via the Nrf2/ARE pathway. (A) Cell viability of KGN cells after treatment with different concentrations of ICA for 24 h. (B), Detection of KGN cell viability treated by ICA or/with cisplatin. (C), Effect of ICA on Cis-induced toxicity in KGN cells validated by LDH assay. (D), Effect of ICA on cis-induced ROS levels in KGN cells detected by flow cytometry. (E), Geometric mean fluorescence intensity was employed for quantitative analysis of ROS levels. (F), The expression levels of Nrf2, HO-1 and Gpx4 proteins in KGN cells of each treatment group were detected by WB. (G–I), Quantification of Nrf2, HO-1, and Gpx4 protein levels.(J–K), Assessment of intracellular lipid peroxidation by flow cytometry using BODIPY C11 staining (J) and quantitative analysis (K). (L), Determination of the level of MDA in each group of KGN cells. Data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, non-statistically significant.

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