Figure 4
Hepatic deletion of Dsg2 or Dsc2 is not affecting metastatic cell attachment. (a, b) Depict the results of mouse perfusion experiments with injection of AsPC-1 control (Ctrl) or DSG2 knockout (KO) cells into the livers of transgenic mice with either a hepatic KO of Dsg2 (Dsg2fl/fl; Alb-Cretg, Dsg2 KO) or Dsc2 (Dsc2fl/fl; Alb-Cretg, Dsc2 KO). Dsg2fl/fl or Dsc2fl/fl mice, respectively, served as control. Values of KO livers were normalized to the respective control livers. Striped bars represent Dsg2 KO livers and squared bars Dsc2 KO livers. The number of tumor cells per perfused liver cells was determined using flow cytometry. (a) AsPC-1 control n = 6 (Control) and 4 (Dsg2 KO) livers, AsPC-1 DSG2 KO n = 4 (Control) and 4 (Dsg2 KO) livers, both two-tailed Mann–Whitney test vs. respective control liver. (b) AsPC-1 control n = 4 (Control) and 5 (Dsc2 KO) livers, two-tailed unpaired t test with Welch’s correction vs. respective control liver. AsPC-1 DSG2 KO n = 4 (Control) and 5 (Dsc2 KO) livers, two-tailed unpaired t test vs. respective control liver.
