Table 1 Sequences of primer and probe.
Assay | Name | Sequence (5′–3′) and modification | Length (bp) |
|---|---|---|---|
Real-time MIRA | lytA-F1 | CTGAGACCTATGCAGCGGTTGAACTGATTG | 30 |
lytA-F2 | CTGATTGAAAGCCATTCAACCAAAGAAGAG | 30 | |
lytA-F3 | CATTCAACCAAAGAAGAGTTCATGACGG | 30 | |
lytA-F4 | TTCATGACGGACTACCGCCTTTATATCG | 29 | |
lytA-R1 | TCACGGCTAATGCCCCATTTAGCAAGATATG | 31 | |
lytA-R2 | TCATGCTTAAACTGCTCACGGCTAATGCC | 29 | |
lytA-P1 | CAGGGAGTTTAGCTGGAATTAAAACGCACGAG[FAM-dT] A[THF][BHQ1-dT] GCACGAATAACCAAC[C3Spacer] | 80 | |
Real-time PCR | lytA-F | ACGCAATCTAGCAGATGAAGCA | 23 |
lytA-R | TCGTGCGTTTTAATTCCAGCT | 21 | |
lytA-P | FAM-TGCCGAAAACGCTTGATACAGGGAG-BHQ1 | 34 |
