Figure 4 | Scientific Reports

Figure 4

From: Bitter tastants relax the mouse gallbladder smooth muscle independent of signaling through tuft cells and bitter taste receptors

Figure 4

TRPM5 is not involved in [Ca2+]i responses of isolated gallbladder smooth muscle cells (CCK+) to quinine and dextromethorphan. Confocal laser scanning recordings of fluorescence intensity of the calcium indicator Fluo-4 fluorescence in CCK+ cells. (A,C) Relative frequencies of responders (> 10% increase in [Ca2+]i; dark column) and non-responders (light column) to increasing concentrations of quinine and dextromethorphan (DXM) in cells from Trpm5 wildtype (Trpm5+/+) and gene-deficient (Trpm5–/–) mice. Chi-square test shows no significant difference between genotypes, p-values are indicated; absolute numbers of cells indicated in the columns. (B,D) Maximum increase in [Ca2+]i in responders (cells that exhibited > 10% increase in [Ca2+]i to at least one concentration of the respective bitter tastant) to (B) quinine and (D) dextromethorphan (DXM); extent of reaction is not significantly different between genotypes (Trpm5+/+ versus Trpm5–/–); Mann–Whitney-U-test, p-values are indicated; n refers to number of cells/number of gallbladders from which they were taken.

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