Figure 1 | Scientific Reports

Figure 1

From: Renogrit selectively protects against cisplatin-induced injury in human renal tubular cells and in Caenorhabditis elegans by harmonizing apoptosis and mitophagy

Figure 1

HPTLC profile of phytochemicals present in Renogrit. (a) Developed HPTLC plate under exposure to UV light (λ = 254 nm) with Retardation factor (Rf) of Gallic acid (GA), Methyl gallate (MG), Renogrit (RENO), Quercetin (QT), and Bergenin (BG). (b) 3D- HPTLC chromatogram of separated peaks showing presence of Renogrit’s marker compounds, GA (Rf = 0.36), MG (Rf = 0.52), QT (Rf = 0.57), and BG (Rf = 0.18) acquired at 280 nm using toluene: ethyl acetate: methanol: formic acid (5:4:1:0.2, by volume) as a developing system using densitometric scan. Quantitative analysis of the marker compounds in Renogrit was done based on the linearity plot and peak tables of (c) GA, (d) BG, (e) MG, and (f) QT along with RENO attained at 280 nm. Quantification of the phytochemicals has been mentioned in Table 1.

Back to article page