Fig. 1
From: BUB1 induces AKT/mTOR pathway activity to promote EMT induction in human small cell lung cancer
SCLC tumors exhibit high BUB1 expression. (A, B) Levels of BUB1 mRNA in normal tissues and SCLC tumors from the GSE30219 (A) and GSE149507 (B) datasets. (C) After using the R limma package to remove batch effects, the GSE30219 and GSE149507 datasets were merged, and BUB1 mRNA levels were compared between patients without (M0) and with (M1) distant metastases. (D) Western immunoblotting revealed the upregulation of BUB1 in SCLC cell lines (SBC-2, NCI-H446, H69AR, DMS114) as compared to control epithelial cells (16HBE). (E) IHC staining for BUB1 in tumors and paracancerous tissues (200 ×), revealing significantly higher IRS scores for tumor samples. *For each experiment, the data are expressed as means ± SD (n = 3). *P < 0.05, **P < 001, ***P < 0.001.
