Fig. 2
From: BUB1 induces AKT/mTOR pathway activity to promote EMT induction in human small cell lung cancer
In vitro analyses of the functional role of BUB1. (A) An overview of BUB1 expression in SCLC cell lines was conducted with the online DepMap tool. The BUB1 expression dataset is shown to the left. In contrast, the CRISPR dataset on the right demonstrates that BUB1 silencing impairs cell growth, with negative scores indicating slower growth following gene knockout. In contrast, positive scores indicate more rapid growth after knockdown. Each gray dot corresponds to a particular cell line. (B) qPCR assessed BUB1 expression in NCI-H446 and SBC-2 cells following its siRNA-mediated silencing. And Western immunoblotting was utilized to confirm decreases in BUB1 protein content following its siRNA-mediated silencing. (C) For assessing the impact of BUB1 knockdown on tumor cell viability, CCK-8 tests were employed. (D) The effect of silencing BUB1 on tumor spheroid growth was measured. (E) The impact of BUB1 silencing on the cell cycle development in SBC-2 and NCI-H446 cells was evaluated using flow cytometry.
