Fig. 3 | Scientific Reports

Fig. 3

From: YTHDF1-regulated ALOX5 in retinal pigment epithelial cells under hypoxia enhances VEGF expression and promotes viability, migration, and angiogenesis of vascular endothelial cells

Fig. 3

ALOX5 depletion decreases VEGF expression induced by hypoxia in ARPE-19 cells. (A) qRT-PCR of ALOX5 mRNA level in ARPE-19 cells after transfection by ALOX5-shRNA1, ALOX5-shRNA2, ALOX5-shRNA3, or shNC lentiviral vector. (B and C) ARPE-19 cells were introduced with shALOX5 or shNC lentiviral vector before hypoxia exposure or normoxia culture and assayed for VEGF expression by immunofluorescence (B) and VEGF secretion level by ELISA (C). *P < 0.05, **P < 0.01, ***P < 0.001.

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