Fig. 3
Effects of biological variables on founder and GLT rates for null allele production. (a) Violin plot of founder rates of cellular non-essential and essential genes. Reported are the p-value (p) of the pairwise comparison using the Wilcoxon rank sum test, the number of genes (N), and corresponding medians in boxplots. (b) Violin plot for founder rates of homozygous lethal and non-lethal genes. Reported are the p-value (p) of the pairwise comparison using the Wilcoxon rank sum test, the number of genes (N), and corresponding medians in boxplots. (c) Stacked bar chart showing GLT of null alleles for essential and non-essential genes with multiple attempts to produce a null allele. (d) Logistic regression model showing the association of each variable with the success of the attempt to generate founders. An odds ratio below 1 is associated with a reduced probability of success, an odds ratio above 1 is associated with an improved probability of success, and an odds ratio of 1 is associated with no effect on success. Table 2 has the odds ratios and p-values for each variable, with and without essentiality in the model, that assess the significance of the difference of the estimate from zero. Supplementary Table 5 has the full model output. Each attempt represents a unique gene with the first attempt that successfully generated the desired allele or the last unsuccessful attempt for each gene used for analysis. See materials and methods for a complete description of data filtering.
