Fig. 2

Squalene monooxygenase (SQLE) is an aberrant metabolic gene overexpressed in ovarian cancer (A) SQLE protein expression levels were assessed by western blot in normal and cancer ovarian tissues and quantified by ImageJ software with grayscale values. (B) There was a statistically difference of p < 0.05 in SQLE between normal and cancer ovarian tissues. (C) The expression of SQLE in benign ovarian lesions, ovarian junctional tumors, and OC was detected by immunohistochemistry. (D, E) in human normal ovarian epithelial cell line (IOSE-80) and 6 OC cell lines by qRT-PCR and western blot. SQLE levels in the six OC cell lines. *P < 0.05, **P < 0.01, #P < 0.001. (F, G, H) The efficiency of knockdown and overexpression of SQLE in three ovarian cancer cells was verified by qRT-PCR 、western blot and immunofluorescence methods.