Fig. 1

Long-lasting behavioral changes induced by socialization depends on memory-related genes. (A) Scheme of the socialization protocol: recently eclosed animals were either grouped or isolated for five days, and subsequently tested. (B) Quantification of food consumption of wt, rut and dnc mutant flies in socialized and isolated conditions (single fly CAFE assay) in the 0–24 h time window (Kruskal-Wallis chi-squared = 75.905, df = 5, p-value = 6.022e-15; post hoc Dunn comparisons: wt^social|wt^isolatedp = 6.24e-13, rut^social|rut^isolatedp = 1.00, dnc^social|dnc^isolated = 1.00). (C) Scheme of the modified socialization protocol: 5-day grouped or isolated animals were isolated for 24 h before testes. (D) Quantification of food consumption of wt, rut and dnc mutant flies in socialized and isolated conditions (sCAFE) in the 24–48 h h time window (Kruskal-Wallis chi-squared = 32.698, df = 5, p-value = 4.32e-06; post hoc Dunn comparisons: wt^social|wt^isolatedp = 1.04e-03, rut^social|rut^isolatedp = 1.00, and, in fig S1, dnc^social|dnc^isolated = 1.00). (E) Sleep profile and (F) sleep quantification of the 24–28 h time window for wt and rut mutant background (Kruskal-Wallis chi-squared = 94.165, df = 3, p < 2.2e-16; post hoc Dunn comparisons: wt^social|wt^isolatedp = 1.07e-02, rut^social|rut^isolatedp = 0.438). (G) Sleep quantification of the 24–28 h time window for wt and dnc mutant background (Kruskal-Wallis chi-squared = 36.476, df = 3, p-value = 5.94e-08; post hoc Dunn comparisons: wt^social|wt^isolatedp = 3.43e-02, dnc^social|dnc^isolatedp = 3.48e-03).