Figure 2

Results of relative mRNA and protein abundance of FAK, Src, MMP-2, MMP-9 and COL-IV in RFM and healthy dairy cows. Note: (A) Western blot analysis of FAK, Src, MMP-2, MMP-9 and COL-IV. (B) Protein expression of FAK, Src, MMP-2, MMP-9 and COL-IV. (C) mRNA expression of FAK, Src, MMP-2, MMP-9 and COL-IV. R: RFM cows. N: healthy dairy cows, N=3, RFM=3. Groups were compared using one-way ANOVA with a Duncan correction. This experiment was repeated 3 times, and the data presented are the mean ± SEM; * P ≤ 0.05, ** P≤ 0.01. Results of mRNA and protein abundance of FAK, Src, MMP-2, MMP-9 and Col-IV in cow endometrial epithelial cells after 24 h of FAK inhabit 14 treatment (A and B: protein abundance level after FAK inhabit 14 treatment; C: mRNA expression level after FAK inhabit 14 treatment).