Fig. 2
Tumor branching evolution reveals intratumor heterogeneity in high-grade serous ovarian cancer. (A) Tumor phylogenetic tree constructed by hierarchical clustering of all the clusters from 14 tumors, in which BR1, BR2, and BR3 were defined according to the hierarchical relationship. (B) Bar plot showing enrichment analysis using the tumor branch evolution features via clusterProfiler. FOR: Formation, PRO: proliferation, ORG: organization, POS REG: positive regulation. (C) Bar plot showing the sample origins of three subtypes of branching evolution. (D) Distribution characteristics of intratumoral cell types obtained through tumor evolutionary analysis. Profile and uniform manifold approximation and projection (UMAP) plots showing the cell-type subgroups in the epithelial, CAF, macrophage, and CD8 cells (top). Velocity and single-cell trajectory results (rows 2 and 3) and differentially expressed genes (rows) along the pseudo-time (columns) were clustered hierarchically into five groups in the scRNA-seq dataset. Pathway enrichment scores were calculated using clusterProfiler.
