Fig. 1
From: Targeted imaging of pulmonary fibrosis by a cyclic peptide LyP-1
The LyP-1 peptide binds to fibrotic lung tissue in BLM-injured mice. (a) Lung tissues were harvested from the BLM instilled mice at day 1, day 5 and day 18. Representative HE-stained and PSR-stained lung sections at day 1, day 5 and day 18 after BLM instillation. (n = 3 ~ 6 mice per group). (b) Mice were i.v. injected with FITC-labeled ARA or LyP-1 peptides at day 18 after BLM instillation. Peptides were allowed to circulate for 2 h, and lungs were harvested after perfusion for frozen sections. Peptides in the lung sections of control or BLM instilled mice were visualized by direct observation of FITC fluorescence (green). (c) Peptides in the lung sections of BLM instilled mice were further visualized by immunohistochemical staining with anti-fluorescein antibody (anti-FITC; red) and were counterstained with 4,6-diamidino-2-phenylindole (DAPI; blue). The threshold was equally enhanced for all samples using ImageJ. Representative sections of ARA or LyP-1 peptide binding to lung tissue of control mice or BLM mice are shown (n = 4 ~ 7 mice per group). Scale bar = 100 µm.
