Fig. 2 | Scientific Reports

Fig. 2

From: Hypoxia-induced TPC2 transcription and glycosylation aggravates pulmonary arterial hypertension by blocking autophagy flux

Fig. 2

The expression of TPC2 was enhanced and autophagic flux was blocked in hypoxic PASMCs. HPASMCs and RPASMCs were divided into normoxia, hypoxic and hypoxic + BafA1 groups. (A and B) Cell migration and proliferation analysis (n = 3). (C) The expression of LC3 and p62 was detected through western blot. (D) TEM examination of autophagy. Scale bar, 1 µm. (E) Autophagic flux was analyzed via mRFP-GFP-LC3 transfection. Red indicates mRFP, and Green indicates GFP. Scale bar, 20 µm. (F) Measurement of lysosomal Ca2+. (G and H) qRT-PCR and western blot analysis of TPC2 expression (n = 3).*P < 0.05, **P < 0.01 and ***P < 0.001. Original blots/gels are presented in Supplementary Fig. 1–3.

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