Fig. 5 | Scientific Reports

Fig. 5

From: Hypoxia-induced TPC2 transcription and glycosylation aggravates pulmonary arterial hypertension by blocking autophagy flux

Fig. 5

Hypoxia induced STT3B-mediated TPC2 glycosylation. (A and B) TPC2 glycosylation in PASMCs treated with normaxia, hypoxia or hypoxia + PNGase F was determined by western blot. (C and D) The expression of STT3A and STT3B in STT3A or STT3B-silencing PASMCs was analyzed by western blot. (E and F) TPC2 glycosylation in STT3A or STT3B-silencing PASMCs was determined by western blot. (G) The putative glycosylation site of TPC2 was highly conserved in human, nonhuman primates and other mammals and shown in red. *P < 0.05, **P < 0.01 and ***P < 0.001. Original blots/gels are presented in Supplementary Fig. 4–8.

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