Fig. 6 | Scientific Reports

Fig. 6

From: Hypoxia-induced TPC2 transcription and glycosylation aggravates pulmonary arterial hypertension by blocking autophagy flux

Fig. 6

STT3B-dependent TPC2 glycosylation inhibited autophagic flux and enhanced migration in hypoxic PASMCs. Human and rat PASMCs were divided into OE-TPC2, OE-TPC2 + si-STT3B and OE-TPC2 + PNGaseF groups and treated with hypoxia. (n = 3) (A) TEM examination of autophagy. Scale bar, 1 µm. (B) Measurement of lysosomal Ca2+. (C) Autophagic flux was analyzed through mRFP-GFP-LC3 transfection. Red indicates mRFP, and Green indicates GFP. Scale bar, 20 µm. (D) Cell migration was assessed by transwell assays. *P < 0.05, **P < 0.01 and ***P < 0.001.

Back to article page