Fig. 6
Effects of structurally different arsenicals on apoptotic cell death. (A) TUNEL assay of skin sections from vehicle- or treated mice revealed abundant TUNEL-positive (green) cells in DPCA and DPCYA-treated skin, indicating robust cell death (Yellow arrows) at 24 and 72 h. Scale bar-50 µm. (B) Histogram showing quantitative analysis of TUNEL-positive green cells. Keyence Microscope inbuilt software (Model BZ-X710, KEYENCE, Osaka, Japan) was used for quantitative analysis of TUNEL-positive cells. Multiple microphotographs captured at 10X magnification was used for quantitative analysis. (C) Western blot analysis shows augmented expression of cleaved caspase 3 in arsenical-challenged mice. β-actin was used as an endogenous control. (D) Histogram representing densitometry analysis of western blots band intensity. (Also see supplementary fig. S4, S5 and S6 for full images of immunoblots). *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001 showing significance compared to vehicle treated controls. ns, non-significant. N = 3/group.
