Fig. 4 | Scientific Reports

Fig. 4

From: A novel lncRNA AC112721.1 promotes the progression of triple-negative breast cancer by directly binding to THBS1 and regulating miR-491-5p/C2CD2L axis

Fig. 4

AC112721.1 functioned as a miR-491-5p sponge in MDA-MB-231 cells. (A) The relative luciferase activities were detected in 293T cells co-transfected with miR-429 mimic or NC mimic and AC112721.1 WT or AC112721.1 MUT. (B) The expression efficiency of mimic-miR-491-5p was assessed using qRT-PCR. (C) Overexpression of miR-491-5p inhibited cell proliferation. (D) Overexpression of miR-491-5p promoted cell apoptosis. (E) Overexpression of miR-491-5p inhibited cell migration. (F) The knockdown efficiency of inhibitor-miR-491-5p was assessed using qRT-PCR. (G) Knockdown of miR-491-5p promoted cell proliferation. (H) Knockdown of miR-491-5p inhibited cell apoptosis. (I) Knockdown of miR-491-5p promoted cell migration. (J) The cell viability of MDA-MB-231 cells treated with pcDNA-AC112721.1 and mimic-miR-491-5p. (K) The cell migration ability of MDA-MB-231 cells treated with pcDNA-AC112721.1 and mimic-miR-491-5p. ANOVA was used for statistical difference analysis. All experiments were performed at least thrice with triplicate samples. **p < 0.01, ***p < 0.001, ****p < 0.0001.

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