Fig. 2
(A) Schematic of 2D single-cell and 3D spheroid models for co-culturing DC-CIK and GBM cells in a transwell system. (B) BTSC233 (stem cells and differentiated cells) were cultured at a 10:1 effector: target ratio for 24 h comparing regular cytotoxicity and transwell cytotoxicity. The cytotoxic effect of DC-CIK cells was measured by flow cytometry. (C) DC-CIK cells on the apoptosis of GBM cell lines (84 and G35) by the Flow Cytometry assay. Flow cytometry figure of changes in the proportion of early apoptosis cells and late apoptosis or necrosis cells. Cells were stained with FITC Annexin V and Percp 7AAD. The result is one of the representative data. (D) IFN-γ and TNF-α secretion in DC-CIK cells targeting GBM cell lines 84, G35, and BTSC233. (E) Relative mRNA expression changes of Wnt/beta-catenin pathway-related genes on BTSC233 (spheroid) cell line after coculturing with DC-CIK cells (E: T ratio 1:1) for 24 h. The results represent data from three separate experiments and are presented as mean ± SD. Significance levels were determined using two-way ANOVA with Bonferroni’s post-hoc test (*P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
