Fig. 2

Double immunofluorescent characterization of αSMA labeling in the human hippocampal mossy fibers and vascular cells. Information about antibody combination, fluorescent indicators, image panel arrangement and scale bars are provided. A complete colocalization of ɑSMA and β-secretase 1 (BACE1) labeling is seen at the mossy fiber terminals (mf) (A, A1, A2, A3). Note the unlabeled mossy cells (*) in the hilus. (B, B1, B2, B3) αSMA labeled MF terminals occur in close proximity to the thorny excrescences of sortilin labeled hilar mossy cells (*) and CA3 pyramidal neurons. (A3, B3) αSMA immunolabeling of the nucleated pericytes at capillaries and small vascular profiles. (C) Typical ɑSMA labeling in the smooth muscle cells in the tunica media (TM) in a section of the basal artery. The internal elastic laminae (IEL) exhibits autofluorescence through the blue and green fluorescence channels. Other abbreviations are as defined in Fig. 1.