Fig. 1 | Scientific Reports

Fig. 1

From: MiR-125b-5p ameliorates ventilator-induced lung injury in rats by suppressing ferroptosis via the regulation of the Keap1/Nrf2/GPX4 signaling pathway

Fig. 1

Ferroptosis is involved in the progression of VILI in rats. (A) Observation of the lung tissue with the naked eye. (B) Wet/dry weight ratio of the lung tissue. (C) Total protein content in BALF. (D) Lung tissue HE staining (200 × and 400 ×) based on observation with a light microscope and lung injury score. (E) Protein expression of GPX4, SLC7A11 and ACSL4 were detected by western blotting, with β-actin as the internal control. (F) DAB-enhanced Perls’ Prussian blue staining was used to evaluate the content of lung tissue ferric ions. Detection of biochemical indicators of ferroptosis: (G) GSH, (H) MDA, (I) Tissue iron. (J) Immunofluorescence microscopy analysis of the distribution of fluorescence labeled GPX4 protein. (***P < 0.001. ****P < 0.0001.) VILI, ventilator-induced lung injury; BALF, bronchoalveolar lavage fluid; HE, Hematoxylin and eosin; ACSL4, Acyl-CoA synthetase long-chain family member 4; GPX4, Glutathione peroxidase-4; SLC7A11, Solute carrier family 7 member 11; GSH, Glutathione; MDA, malondialdehyde.

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