Fig. 13 | Scientific Reports

Fig. 13

From: MiR-125b-5p ameliorates ventilator-induced lung injury in rats by suppressing ferroptosis via the regulation of the Keap1/Nrf2/GPX4 signaling pathway

Fig. 13

MiR-125b-5p inhibits ferroptosis by targeting the Keap1/Nrf2/GPX4 axis, thereby alleviating MS-induced injury cell. (A,B) expression of miR-125b-5p and Keap1 mRNA in the different groups of MS-induced injury cell models were determined by qRT-PCR. (C,D) Protein expression of Keap1, GPX4, SLC7A11 and ACSL4 were detected by western blotting, with β-actin as the internal control. (E) Protein expression level of Nrf2 was measured by western blotting, with Histone H3 as the internal control. (**P < 0.01. ***P < 0.001. ****P < 0.0001). MS, mechanical stretch; qRT-PCR, real-time quantitative polymerase chain reaction; Keap1, Kelch-like epichlorohydrin-associated protein 1; GPX4, Glutathione peroxidase-4; SLC7A11, Solute carrier family 7 member 11; ACSL4, Acyl-CoA synthetase long-chain family member 4; Nrf2, Nuclear factor-erythroid2-related factor 2.

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