Fig. 8 | Scientific Reports

Fig. 8

From: MiR-125b-5p ameliorates ventilator-induced lung injury in rats by suppressing ferroptosis via the regulation of the Keap1/Nrf2/GPX4 signaling pathway

Fig. 8

MiR-125b-5p alleviated ferroptosis via upregulating GPX4 in in MS-induced injury cell models. (A) concentration gradient for the GPX4 inhibitor RSL3 by western blotting. (B) Protein expression levels of SLC7A11, GPX4 and ACSL4 in MS-induced injury cell were measured by Western blotting, with β-actin as the internal control. (C) ROS detected by immunofluorescence in MS-induced injury cell model. Green: ROS. Magnification: 40×. Detection of biochemical indicators of GSH (D), MDA (E) and total iron (F) in MS-induced injury cell models. *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001. MS, mechanical stretch; SLC7A11, Solute carrier family 7 member 11; GPX4, Glutathione peroxidase-4; ACSL4, Acyl-CoA synthetase long-chain family member 4; ROS, Reactive oxygen species; GSH, Glutathione; MDA, malondialdehyde.

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