Fig. 6 | Scientific Reports

Fig. 6

From: Effect of defective H2B ubiquitination on the malignancy and repair of DNA double breaks and its significance in lung adenocarcinoma

Fig. 6

Chemotherapy Sensitivity of RNF20-Silenced A549 Cells at Various Drug Concentrations. (a) Olaparib treatment (0, 5, 10, 20 µg/ml) decreased viability dose-dependently in​​ Control shRNA-A549 and RNF20 shRNA-A549 cells (assessed by the trypan blue exclusion assay). ​​RNF20 silencing conferred enhanced sensitivity at all concentrations.​​ ​​(b) Cisplatin treatment (0.5, 1, 2, 4 µM for 72 h) also significantly reduced viability in both groups, with a stronger effect in​​ RNF20-silenced cells.(c) Combination treatment with Olaparib (20 µg/ml) and Cisplatin (2 µM) Data are presented as mean ± SEM, and significance was determined using a two-way ANOVA. Magnification: 10×, scale bar: 100 μm. (**P < 0.01)

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