Fig. 4
From: Histone modification changes upon exposure of human lung adenocarcinoma cells to nanoparticles
Histone H3 methylation after treatment with NPs. (A) Tri-Me-H3K4, Di-Me-H3K9 and Tri-Me-H3K27 after treatment with NPs. A549 cells were treated with NPs (0.01 mg/mL) for various time points. H3 (CBB staining) was used as a standard for the equal loading of proteins for SDS-PAGE; (B) Tri-Me-H3K4, Di-Me-H3K9 and Tri-Me-H3K27 after treatment with CuO-NPs; (C) Tri-Me-H3K4, Di-Me-H3K9 and Tri-Me-H3K27 after treatment with ZnO-NPs. Tri-Me-H3K4, Di-Me-H3K9 and Tri-Me-H3K27 were determined with western blotting, in which the intensity of each band was extracted using ImageJ version 1.38. The extent of Tri-Me-H3K4, Di-Me-H3K9 or Tri-Me-H3K27 in cells treated with CuO-NPs, ZnO-NPs or TiO2-NPs versus untreated control cells was calculated. All the above blots had already been cropped in order to improve the clarity and conciseness of the presentation. The original blots are presented in Supplementary Fig. 7.
