Fig. 2 | Scientific Reports

Fig. 2

From: Yiqi Juanshen decoction alleviates renal interstitial fibrosis by targeting the LOXL2/PI3K/AKT pathway to suppress EMT and inflammation

Fig. 2

YQJSD alleviates UUO-induced renal fibrosis. (A) Masson staining and immunohistochemistry were used to evaluate renal collagen deposition (scale bar = 100 μm, n = 6) and protein levels of COL4α1, FN, and α-SMA (scale bar = 50 μm, n = 6). (B) Collagen volume fractions were determined via Masson staining (n = 6). (C–D) Protein levels of COL4α1 and FN were quantified (n = 6). (E–F) Relative mRNA levels of COL4α1 and FN in kidney tissues were assessed by qPCR (n = 3). (G) Protein levels of α-SMA were quantified (n = 6). (H) α-SMA protein levels in renal tissues via Western blot (n = 3). (I) Quantification of α-SMA protein signal from (H) (n = 3). (J) Relative α-SMA mRNA levels by qPCR (n = 3). (K) Serum TGF-β1 levels via ELISA (n = 8). (L) Relative TGF-β1 mRNA in renal tissues by qPCR (n = 3). Data are shown as mean ± SD. P-values were calculated using One-way ANOVA with Tukey’s test. ##p < 0.01 and ###p < 0.001 vs. Sham group. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. UUO group.

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