Fig. 1 | Scientific Reports

Fig. 1

From: Influence of experimental variables on spheroid attributes

Fig. 1

Standardizing 3D spheroid culture: key experimental factors and analysis framework. Standardizing 3D spheroid culture requires optimizing key parameters such as medium composition, oxygen concentration, and FBS levels to ensure consistent formation and growth. Advanced spheroid analysis, including AI-driven tools and automated screening, enhances characterization and reproducibility. Transparent reporting and open-access databases further improve data sharing, facilitating translational applications in drug screening and regenerative medicine. Created with BioRender.com by Songshan Zhu.

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