Fig. 2
The deficiency of YTHDC1 suppresses the proliferation of colorectal cancer cells and increases apoptosis. (a) Proliferate curve of HCT116 and SW480 cells after knocking down YTHDC1. Data was analyzed by CCK8 with OD 450 nm. (b) Western blot analysis of YTHDC1 expression in HCT116 and SW480 cells after knocking down YTHDC1. (c) Colony formation analysis of HCT116 and SW480 cells after knocking down YTHDC1. The scale bar is 5 mm. (d) Quantification of colony numbers from Fig. 2c. (e) Effects of WT-YTHDC1, YTHDC1-W327A, or YTHDC1-W428A overexpression in YTHDC1-knocking down HCT116 cells. mCherry and siNC were the control for overexpressing plasmids and siRNAs. Cells were counted at day 2 after being transfected for 24 h. Synonymous mutations were introduced into the siRNA-targeting sequences of these plasmids to ensure the overexpression of exogenous genes. (f) Quantification of annexin V-APC positive cell percentage in Flow cytometry analysis after knocking down YTHDC1. (g) Growth curve of xenograft tumors from HCT116 cells. n = 5. (h) Xenograft tumors from HCT116 cells. (i) Quantification of tumor weight from Fig. 2F. n = 5. Data are presented as the mean ± SD of three independent experiments (a,d,e,f). *p < 0.05, **p < 0.01, ***p < 0.001; NS no significance; two-tailed Student’s t test.
