Fig. 7 | Scientific Reports

Fig. 7

From: Early stage of metabolic dysfunction associated steatotic liver disease disrupts circadian rhythm and induces neuroinflammation in rats

Fig. 7

Immunofluorescence staining for microglia and astrocyte in frontal cortex and cerebellum. Representative images (20x) of microglia (Iba1) staining in control group and HFHC group for frontal cortex (a) and cerebellum (b). Number of cellular bodies per mm2 and total ramification area per frame area of Iba1 stained cells in frontal cortex (c) and cerebellum (d). Representative images (10x) of astrocyte (GFAP) staining in control group and HFHC group for frontal cortex (e) and cerebellum (f). Region areas detected as astrocytes/frame area and Average Intensity per Region (AIR)/Average Intensity value of Channel ‘AF488’ (AIC) of GFAP stained cells in frontal cortex (g) and cerebellum (h). Data are expressed as mean ± SD or median (IQR). Unpaired Student t-test or Mann–Whitney test: *p < 0.05 compared to control group (n = 3–4 per group).

Back to article page