Fig. 6
Myeloid cells subpopulation analysis found CHIT1 mainly expressed in neutrophils and CHIT1 + neutrophils highly accumulated in sepsis non-survivors. (A) UMAP plot showed myeloid cells could be grouped into 4 distinct clusters based on their gene expression profiles, each represented by a different color. (B) These 4 distinct clusters were annotated as D14 + + monocytes/macrophages, neutrophils, dendritic cells (DC), and CD14 + monocytes/macrophages with the help of SingleR. The clusters were annotated with their respective cell type labels, providing a clear visualization of the distribution and separation of different cell types. (C) Heatmap showing the expression levels of subpopulation specific marker genes across these 4 different immune cell types. The cell types were color-coded at the top of the heatmap. The heatmap was divided into six clusters C1 to C4, each representing a group of genes with similar expression patterns. The color gradient from blue to red indicated low to high expression levels, respectively. The genes were listed on the right side, with some genes highlighted in red for emphasis. (D) Violin plot displaying the expression levels of the CHIT1 gene across these 4 different immune cell types. This scatter plot showed the expression levels of the CHT1 gene across various cell types. The x-axis represented different cell types, and the y-axis represented the expression level of CHT1. Each point represented a single cell, and the color of the points corresponds to the cell type, as indicated by the legend. (E) Bar plot depicted the relative proportions of these 4 different immune cell types in different disease status. This comparison highlighted CHIT1 + neutrophils was highly accumulated in sepsis related death patients.
