Extended Data Fig. 2: PPGNTS neurons suppress eating without behavioural disruption.
From: Central and peripheral GLP-1 systems independently suppress eating
a, Non-cumulative hourly food intake over the circadian cycle from ad libitum eating PPGNTS-hM3Dq mice (n = 7 animals for analyses presented in a-b), 2-way within-subjects ANOVA: Drug x Time F(23,138)=4.599, p < 0.0001. b, Dark phase food intake by sex, 2-way mixed-model ANOVA: Drug F(1,5)=19.97, p = 0.0066; Sex F(1,5)=3.854, p = 0.107. c,d, Photomicrographs of colocalized cFos immunoreactivity and DIO-hM3Dq-mCherry in NTS of PPG-Cre:tdRFP mice perfused 3 hours after injection of saline or CNO (photomicrographs representative of independent experiments from 4/3 animals), cc: central canal. Scale=100 µm (inset 50 µm). e, Proportion of mCherry-expressing neurons co-localised with cFos-ir in mice perfused after administration of saline or CNO (n = 4 (SAL) / 3 (CNO) animals), unpaired 1-tailed t-test: t(5)=13.94, p < 0.0001. f, Non-cumulative hourly food intake over 1 day from 18 h fasted PPGNTS-hM3Dq mice (n = 7 animals for analyses presented in f-h), 2-way within-subjects ANOVA: Drug x Time F(23,138)=3.745, p < 0.0001. The behavioural satiety sequence (BSS) was analysed during the first 40 minutes of the dark phase. g,h, Food intake and eating rate over 40 minute BSS test, paired 2-tailed t-test and Wilcoxon matched pairs test: (g) t(6)=4.088, p = 0.0064; (h) W = 18, p = 0.156. All data presented as mean ± SEM.
