Extended Data Fig. 6: PPGNTS neurons are not a major synaptic target of area postrema Glp1r neurons.
From: Central and peripheral GLP-1 systems independently suppress eating
a, Photomicrographs of coronal NTS section showing RABV expression, Glp1r FISH and TH-ir. RABV + Glp1r+TH-ir colocalisation shown by white-edged green arrows (photomicrographs representative of independent experiments from 4 animals). Scale=100μm (inset 20μm). b,c, Quantification of Glp1r and TH-ir co-localization in area postrema (b), and proportions of dual Glp1r / TH-ir cells co-localised with RABV (c). This dual population comprises 49.4% of all TH-ir cells and 31.2% of all Glp1r cells. 9.7% of RABV + AP inputs to PPGNTS neurons express Glp1r and are TH-ir, and 2.7% of dual Glp1r / TH-ir cells are RABV + AP inputs to PPGNTS neurons. All data presented as mean ± SEM.
