Extended Data Fig. 7: Liraglutide and semaglutide suppress eating independently of PPGNTS neurons.
From: Central and peripheral GLP-1 systems independently suppress eating
a-e, Cumulative food intake by virus at 1,2,4,6 and 21 hr in eGFP and DTA mice (n = 8 (DTA) / 7 (eGFP) animals for analyses presented in (a-j) administered liraglutide (200 μg/kg, s.c.), 2-way mixed-model ANOVA: (a) Drug F(1,13)=0.246, p = 0.628; (b) Drug F(1,13)=2.108, p = 0.170; (c) Drug F(1,13)=37.44, p < 0.0001, Virus F(1,13)=0.836, p = 0.377; (d) Drug F(1,13)=75.09, p < 0.0001, Virus F(1,13)=1.877, p = 0.194; (e) Drug F(1,13)=154.9, p < 0.0001, Virus F(1,13)=1.272, p = 0.280. f-j, Cumulative food intake by virus at 1,2,4,6 and 21 hr in eGFP and DTA mice administered semaglutide (60 μg/kg, s.c.), 2-way mixed-model ANOVA: (f) Drug F(1,13)=1.965, p = 0.184; (g) Drug F(1,13)=17.1, p = 0.0012; Virus F(1,13)=0.630, p = 0.442; (h) Drug F(1,13)=82.49, p < 0.0001, Virus F(1,13)=0.332, p = 0.574; (i) Drug F(1,13)=98.21, p < 0.0001, Virus F(1,13)=0.840, p = 0.376; (j) Drug F(1,13)=126.1, p < 0.0001, Virus F(1,13)=3.42, p = 0.0873. k-m, Representative photomicrographs of cFos immunoreactivity (cFos-ir) in arcuate nucleus of the hypothalamus (ARC) 4 hours after vehicle (VEH, n = 4 animals) or semaglutide (SEMA, 60 μg/kg, s.c., n = 4 animals) administration, and total cFos count, unpaired 1-tailed t-test: m) t(6)=2.614, p = 0.020. Scale=100μm. (n-p) Representative photomicrographs of cFos-ir in paraventricular nucleus of the hypothalamus (PVN) 4 hours after vehicle or semaglutide administration (n = 4 / 4 animals), and total cFos count, unpaired 1-tailed t-test: (p) t(6)=5.109, p = 0.0011. Scale=100μm. q-t, Representative photomicrographs of cFos-ir in dorsal lateral and external lateral subdivisions of the parabrachial nucleus (dlPBN / elPBN) 4 hours after vehicle or semaglutide administration (n = 3 / 4 animals), and total cFos count, unpaired 1-tailed t-tests: (s) t(5)=1.693, p = 0.0756; (t) t(5)=3.57, p = 0.0080. Semaglutide did not increase cFos-ir in the medial PBN, t(5)=0.435, p = 0.341. Scale=100μm. All data presented as mean ± SEM.
