Extended Data Fig. 1: PPGNTS neurons selectively encode large meal satiation.
From: Central and peripheral GLP-1 systems independently suppress eating
a, Experimental model and paradigm for metabolic phenotyping of PPGNTS-DTA mice (DTA, n = 8) or mCherry-transduced controls (mCh, n = 7). n = 8 (DTA) / 7 (mCh) animals for analyses presented in b-o. b, Cumulative hourly food intake over 1 day, 2-way mixed-model ANOVA: Virus F(1,13)=0.015, p = 0.904. c, Daily food intake by sex, 2-way mixed-model ANOVA: Virus F(1,11)=0.012, p = 0.914; Sex F(1,11)=0.683, p = 0.426. d, Directed ambulatory locomotion (excluding fine movements) over 1 day, 2-way mixed-model ANOVA: Virus F(1,11)=0.493, p = 0.497. e-i, Meal pattern and metabolic parameters over 1 day, unpaired 2-tailed t-test or Mann-Whitney U test: (e) U = 25, p = 0.779; (f) t(13)=0.997, p = 0.337; (g) t(13)=0.565, p = 0.582; (h) t(13)=0.797, p = 0.440; (i) t(13)=0.323, p = 0.752. j, Mean bodyweight over the 24 h test period, unpaired 2-tailed t-test: t(13)=0.883, p = 0.393. k,l, Food intake during dark and light phases, unpaired 2-tailed t-test: (k) t(13)=0.668, p = 0.516; (l) t(13)=1.251, p = 0.233. m, 24 h water intake, unpaired 2-tailed t-test: t(13)=0.205, p = 0.841. n, Ensure liquid diet preload intake, unpaired 2-tailed t-test: t(8)=0.219, p = 0.832; and post-Ensure chow intake, Mann-Whitney U test: U = 2, p = 0.038. o, Post-fast refeed intake, unpaired 2-tailed t-test: t(12)=2.501, p = 0.028. p-q, Hourly and cumulative intakes over 1 day from ad libitum eating PPGNTS-hM4Di mice (n = 8 animals), 2-way within-subjects ANOVA: (p) Drug F(1,7)=0.241, p = 0.639; (q) Drug F(1,7)=0.411, p = 0.542. r, Raster plot of chow pellet retrievals during the dark phase. Plots from the same mouse after saline and CNO injections presented adjacently. All data presented as mean ± SEM.
