Extended Data Fig. 9: The liver damage in Mettl3ΔHep mice were reduced by Smpd3 knockdown.
From: m6A modification-tuned sphingolipid metabolism regulates postnatal liver development in male mice
a,b, Silencing efficiency of siRNAs targeting Smpd3 was detected by qRT-PCR (n = 4) (a) and immunoblotting (b) in mouse liver cell line AML12. c, qRT-PCR detection of sphingolipid metabolism-related genes in livers from WT (n = 4) and Mettl3ΔHep mice treated with (n = 4) or siSmpd3 (n = 5). d, IHC of MDA, p-eIF2α, CD45 and Epcam from siNC- or siSmpd3-treated livers. (n = 3 biological independent samples). e, Quantification for Atf3, Bax, Mettl3, cl-Casp3 and PCNA proteins in Fig. 8k (n = 3). f, qRT-PCR of livers from WT (n = 4) and Mettl3ΔHep mice treated with siNC (n = 4) or siSmpd3 (n = 5) for indicated genes. Data are shown in mean ± SEM; ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by unpaired two-tailed Student’s t test.
