Extended Data Fig. 6: Depletion of CRAT has no effect on total mitochondrial DNA level in NRVMs and adult CMs.
From: CRAT links cholesterol metabolism to innate immune responses in the heart
a, b, NRVMs were transduced with lentivirus expressing control shRNA or Crat shRNA. Cells were harvested and mitochondria and cytosol fractionation was then performed after 5 days. Western blots indicated that there was no contamination between these two fractions (a). RT-PCRs were then performed with mitochondrial fraction to quantify the total mitochondrial DNA level (b). c, Adult CRAT-WT and CRAT-mKO male and female mice were injected with tamoxifen (20 mg/kg/day) for four consecutive days. Three weeks later, CMs were isolated from hearts for mitochondria and cytosol fractionation. RT-PCRs were then performed with mitochondrial fraction to quantify the total mitochondria DNA level. n = 5 biologically independent samples (b), 6 mice (c) per group. Data are shown as the mean ± SEM. Analysis was unpaired two-tailed Student’s t-test (b-c). NS, not significant.
