Extended Data Fig. 1: Elevated ER and osmotic stress responses in ced-3(-) and ced-4(-) animals.
From: Proteolytic activation of fatty acid synthase signals pan-stress resolution
a-b, representative images (a) and quantification (b) of HSP-4p::GFP stress reporter induction under ER stress at different time points. Wild type and ced-3(-) were repeated from main figure for comparison to ced-4(-) animals. n = 40 animals. c, quantification of ced-4(-) animals escaping larval arrest with tunicamycin (TM) treatment to induce ER stress. n = 3 biological replicates. mean ± SD. d, representative images of NLP-29p::GFP (antimicrobial peptide) stress reporter induction with 400 mM NaCl treatment to induce osmotic stress in ced-3(-) and ced-4(-) animals at different time points. e, digital zoom in of 0 h and 32 h images shown in d of epidermal NLP-29p::GFP reporter induction in ced-3(-) animals under osmotic stress. f, quantification of NLP-29p::GFP stress reporter expression under osmotic stress at early time points. n = 50 animals. g, quantification of NLP-29p::GFP stress reporter expression for both ced-3(-) and ced-4(-) animals. n = 40 animals. For a,d, scale bar, 1 mm. For e, scale bar, 200μm. For b,f,g each circle represents one animal. Mean pixel intensity of each animal was normalized to mean value of wild type at 0 hr (no stress) and plotted as fold change. Violin plots show median (solid line) with quartiles (dashed line). p values were calculated using Mann-Whitney, two-tailed test (b,f,g) and unpaired two-tailed t-test (c).
