Fig. 2: Central inceptor immunoreactivity is restricted to neurons, including those regulating energy and glucose metabolism.
a,b, Representative image (a) and quantification (b) of inceptor immunoreactivity in hypothalamic nuclei of 18- to 24-week-old male C57BL/6J WT mice (n = 30 mice for ARC and DMH, n = 28 mice for VMH, n = 19 mice for PVN). Scale bar, 200 μm. c, Inceptor colocalization with NeuN, Pomc–green fluorescent protein (GFP), Npy–GFP, Aldhl1–GFP, Gfap and Iba1 in the hypothalamus of 28-week-old male C57BL/6J WT mice. Scale bars, 50 μm. d, Inceptor immunoreactivity in 28-week-old chow- versus HFD-fed male C57BL/6J WT mice (n = 5 for all regions except PVN: n = 3). e, Hypothalamic inceptor messenger RNA expression in 10-week-old male C57BL/6J WT mice fed with either chow or HFD (n = 4 per group). f, HOMA-IR in 14-week-old C57BL/6J WT mice fed with either chow or HFD (n = 8). *P < 0.05, Data are presented as mean ± s.e.m. Data in b and e were analysed by one-way ANOVA, data in d were analysed by two-way ANOVA and data in f were analysed using the Kruskal–Wallace test. P values for group differences are: P < 0.0001 (ARC versus VMH), P < 0.0001 (ARC versus DMH), P = 0.0046 (ARC versus PVN), P = 0.0014 (VMH versus DMH), P < 0.0001 (VMH versus PNV), P < 0.0001 (DMH versus PVN) (b), P = 0.0156 (d), P = 00137 (e), P = 0.0217 (chow versus HFD 14 d) and P = 0.0232 (chow versus HFD 28 d) (f). A more detailed statistical report is provided in Source data. Rel., relative.
