Fig. 4: DIO male mice with adult-onset, β cell-specific inceptor deletion show improved glucose metabolism.
a, Inceptor immunoreactivity in the pancreas of male 26-week-old Ins1CreERT−/−Iirflx/flx (WT) and Ins1CreERT+/−Iirflx/flx (KO) C57BL/6J mice. Scale bars, 20 μm. b–e, Body weight (b), as well as lean (c) and fat (d) tissue mass and fasting glucose (e) in 30-week-old male C57BL/6J WT and KO mice (n = 8 for each genotype). Tam, Tamoxifen. f,g, Intraperitoneal glucose tolerance (f) and AUC (g) in 18-week-old male C57BL/6J WT and KO mice on an HFD (n = 8 for each genotype). h,i, Intraperitoneal insulin tolerance (h) and AUC (i) in 18-week-old male C57BL/6J WT and KO mice on an HFD (n = 10 for each genotype). j,k, Fasting levels of plasma insulin (n = 10 for each genotype) (j) and glucagon (k) (n = 10 for each genotype) in 22-week-old male C57BL/6J WT and KO mice. l,m, Pancreatic α cell mass (l; n = 5 each genotype) and β cell mass (m; n = 6 WT, n = 5 KO) in 26-week-old male C57BL/6J WT and KO mice. n, Whole-blood HbA1c in 18-week-old male C57BL/6J WT and KO mice (n = 10 mice per genotype). *P < 0.05, **P < 0.01. Data are presented as mean ± s.e.m. Data in c, d, e, g, l, j, k, l, m and n were analysed by two-sided, two-tailed Student’s t-test, data in b, f and h were analysed by two-way ANOVA with Bonferroni’s post hoc comparison for individual time points and data in n were analysed using Mann–Whitney U-test. P values for group differences are P < 0.0426 (f), P = 0.0322 (g), P = 0.0382 (h), P = 0.0379 (i), P = 0.0142 (m) and P = 0.002 (n). A more detailed statistical report and the P value for the multiple-comparison test (f) are provided in Source data.
